Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p087 | (1) | WCRB2014

Semi-quantification and elucidation of bovine embryo biomarkers by mass spectrometry imaging

Goncalves Roseli F , Ferreira Monica S , de Oliveira Diogo N , Canevarolo Rafael R , Achilles Marcos A , Killian Gary J , Bols Peter E , Visintin Jose A , Catharino Rodrigo R

In the field of ‘single cell analysis’, many classical strategies like immunofluorescence and electron microscopy are the fundamental choices. However, these methodologies are time-consuming and do not permit direct identification of specific molecular classes such as lipids. A novel mass spectrometry-based analytical approach has been applied to bovine oocytes and embryo. This new metabolomics-based application uses mass spectrometry imaging (MSI), efficient data pr...